{"id":77,"date":"2021-11-05T12:57:10","date_gmt":"2021-11-05T11:57:10","guid":{"rendered":"https:\/\/docenciamicrobiologia.umh.es\/?page_id=77"},"modified":"2023-02-02T23:31:10","modified_gmt":"2023-02-02T22:31:10","slug":"temperatura","status":"publish","type":"page","link":"https:\/\/docenciamicrobiologia.umh.es\/en\/indice-de-practicas\/4-influencia-del-medio-ambiente\/temperatura\/","title":{"rendered":"Temperature"},"content":{"rendered":"

Influence of the temperature on microbial growth<\/strong><\/p>\n

Content<\/strong><\/p>\n

Factors that influence the growth of microorganisms: the temperature. Maximum, optimum and minimum temperature. Hyperthermophiles, thermophiles, mesophiles and psychrophiles.<\/p>\n

Overview<\/strong><\/p>\n

The growth of the microorganisms is influenced by various factors. The most important of these include the aeration and the temperature. With regard to the latter, the Temperature<\/strong>, the microorganisms have a range of temperatures in which they can grow. This range is determined by the maximum temperature <\/em>of growth, above which they cannot live and they even die; the minimum temperature<\/em> below which they cannot grow, although in general they do not die; and the optimum temperature<\/em> in which they grow the best.<\/p>\n

<\/p>\n

In view of this temperature range of growth, the microorganisms are classified into:<\/p>\n

Hyperthermophiles:<\/u> Their optimum temperature is above 80\u00baC. Many of them are archaea.<\/p>\n

Thermophiles<\/u>: Their optimum temperature is between 45-70\u00baC. They are usually free-living microorganisms.<\/p>\n

Mesophiles<\/u>: Their optimum temperature is between 25-45\u00baC. \u00a0Pathogenic and commensal microorganisms of humans and warm blooded animals and some free-living microorganisms are included in this group.<\/p>\n

Psychrotrophic:<\/u> The optimum temperature of the microorganisms of this group is below 25 \u2013 30\u00b0C and free-living microorganisms are included in it.<\/p>\n

Psychrophiles<\/u>: Their optimum growth temperature is between 12 \u2013 15\u00b0C.<\/p>\n

\"class=\"<\/p>\n

The methods that are used to obtain a constant temperature for the in vitro culture of the microorganisms are:<\/p>\n

    \n
  1. The culture oven<\/em>. – There might be fluctuations of between 1 to 3\u00baC in it.<\/li>\n
  2. The thermostatic bath<\/em>. – It keeps the temperature more stable. The fluctuations in it are usually less than 1\u00baC.<\/li>\n<\/ol>\n

    Objectives<\/strong><\/p>\n

    Observe the influence that the temperature has on the development of the microorganisms.<\/p>\n

    Carrying out the practical<\/strong><\/p>\n

    Three nutrient agar plates are inoculated with the mixed culture using the inoculation loop streaking technique or the streak plate method.<\/p>\n

    They are incubated at a room temperature (25\u00baC approximately), 4\u00baC, 37\u00baC and 60\u00baC for 24 hours.<\/p>\n

    After incubation it is observed that there was no growth on the plates that were at 4 and 60\u00baC, while growth is observed on the two plates incubated at room temperature and at 37\u00baC, although there was more growth on the plate that was incubated at 37\u00baC. So the conclusion drawn is that the microorganisms in the culture are mesophiles, seeing as their optimum growth is at 37\u00baC, while room temperature, between 22-25\u00baC, is within their growth range seeing as they have grown but less.<\/p>\n

     <\/p>\n

    \n\n\n\n
    \"\"\"\"\"\"<\/p>\n\n\n\n\n
    \"\"<\/td>\n\"\"<\/td>\n\"\"<\/td>\n<\/tr>\n
    After 24 h at 37\u00baC<\/td>\nAfter 24 h at 4\u00baC<\/td>\nAfter 24 h at 60\u00baC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

     <\/p>\n

    To check the effect of the minimum temperature (4\u00baC) and the maximum temperature (60\u00baC) the two latter plates are now incubated at 37\u00baC for 24 hours. After this incubation period it is observed that the microorganisms that were previously subjected to 4\u00baC grow normally while in the plate that had been previously incubated at 60\u00baC there is either no growth or a few colonies might appear, which shows that some species that exist in the mixed culture do produce spores, structures that are resistant to adverse environmental factors, such as the high temperatures.<\/p>\n

    \n\n\n\n\n
    \"\"<\/td>\n\"\"<\/td>\n<\/tr>\n
    Plate at 4\u00baC incubated 24 h at 37\u00baC<\/td>\nPlate at 60\u00baC incubated 24 h at 37\u00baC<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

     <\/p>\n

    Educational Innovation Activities Assistance- Vice Rector for Academic Affairs\/ Enquiries and technical matters – Tel: 96 522 2059<\/strong> – info@goumh.es<\/a><\/p>\n

    <\/p>","protected":false},"excerpt":{"rendered":"

    Influence of the temperature on microbial growth
    \nContent
    \nFactors that influence the growth of microorganisms: the temperature. Maximum, optimum and minimum temperature. Hyperthermophiles, thermophiles, mesophiles and psychrophiles.
    \nOverview
    \nThe growth of the microorganisms is influenced by various factors. The most important of these include the aeration and the temperature. With regard to the latter, the Temperature, the microorganisms have […]<\/p>\n","protected":false},"author":3782,"featured_media":0,"parent":74,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"_links_to":"","_links_to_target":""},"yoast_head":"\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\t\n