{"id":227,"date":"2021-11-05T13:22:28","date_gmt":"2021-11-05T12:22:28","guid":{"rendered":"https:\/\/docenciamicrobiologia.umh.es\/?page_id=227"},"modified":"2022-02-01T19:53:02","modified_gmt":"2022-02-01T18:53:02","slug":"12-preparacion-de-yogur","status":"publish","type":"page","link":"https:\/\/docenciamicrobiologia.umh.es\/en\/12-preparacion-de-yogur\/","title":{"rendered":"Safety Rules"},"content":{"rendered":"<p><strong>GENERAL RULES ON WORKING IN THE MICROBIOLOGY LABORATORY <\/strong><\/p>\n<p>A Microbiology laboratory is a suitable place that is fitted out to handle and examine microorganisms. This type of work must be carried out with a good aseptic technique, and therefore a clean and tidy environment is required and work must always be carried out in sterile conditions (with the flame of a Bunsen burner, alcohol or gas close at hand).<br \/>\nAlthough the microorganisms that are handled are not considered to be pathogenic, all the cultures of all the microorganisms must be handled with care given their potential pathogenicity.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>BASIC CONSIDERATIONS FOR THE WORK:<\/strong><\/p>\n<p>1. Microorganisms under analysis must be kept in their containers and culture mediums to avoid the risk of getting contaminated or contaminating a colleague.<br \/>\n2. Make sure that microorganisms are not in the atmosphere (skin, hair, air, clothes, etc.), which contaminate our samples.<\/p>\n<p>To maintain these conditions, it is necessary to respect a series of<\/p>\n<p>RULES AND RECOMMENDATIONS:<\/p>\n<p>I. On clothing, hygiene and personal belongings (video):<br \/>\n\u00b7 You must go to the laboratory wearing a white coat.<br \/>\n\u00b7 When working close to the flame of the Bunsen burner you must<br \/>\no Avoid wearing loose wide sleeves.<br \/>\no Keep your hair tied back.<br \/>\n\u00b7 When beginning and ending the practicals, students must wash their hands with soap and water.<br \/>\n\u00b7 Books, folders, coats and any other material that is not used when carrying out the practical must be stored properly away from the work place.<br \/>\n\u00b7 Eating, drinking, putting on make-up and obviously smoking in the laboratory is not allowed.<br \/>\n\u00b7 Mobile phones should not be used when carrying out the practicals.<\/p>\n<p>II. On the lab bench:<br \/>\n\u00b7 The workplace must be clean and tidy. Before starting each practical the work surface should be disinfected. The most normal disinfectants are bleach and alcohol (ethanol 96\u00b0).<br \/>\n\u00b7 Microbiology work is mainly done in an atmosphere that surrounds a flame of a Bunsen burner (video and figure 1). It is important to concentrate when working and be careful not to cause a flash fire or get burnt. The Bunsen burner must always be turned off before leaving the lab bench.<br \/>\n\u00b7 At the end of the day\u2019s work, collect the material and clean the table replacing the filter paper if necessary. The students are responsible for looking after the lab bench throughout the practicals.<\/p>\n<p>III. About waste:<br \/>\nThere are different kinds of waste that can be produced in the laboratory. Each type must be disposed of in the corresponding container. There are three basic types of waste:<br \/>\n\u00b7 NON-risk waste, which is disposed of in the conventional rubbish bags.<br \/>\n\u00b7 Biohazardous rubbish, which goes in special containers marked with the biohazard symbol (see figure 2). Nothing contaminated should ever be thrown down the sink or in the normal rubbish.<br \/>\n\u00b7 Glass waste (microscope slides and slide covers, Pasteur pipettes\u2026) go in hard bench top containers (figure 2).<br \/>\n\u00b7 Under no circumstances whatsoever should any sample or material that contains microorganisms (plates, slides, test tubes, etc.) be taken out of the laboratory.<br \/>\n\u00b7 Pipettes must never be put in the mouth. Always use manual pipette controller or automatic pipettes.<br \/>\n\u00b7 If there is an accident (broken material, spilled microorganisms, burns, etc.) the teacher must be informed immediately. There are showers and safety eye wash units (figure 3), as well as special absorbent products for accidental spillages (figure 4).<br \/>\n\u00b7 There must always be a properly equipped first aid kit in the laboratory (figure 5).<\/p>\n<p><img loading=\"lazy\" class=\"aligncenter\" src=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-25.png\" alt=\"\" width=\"780\" height=\"562\" \/><\/p>","protected":false},"excerpt":{"rendered":"<p>GENERAL RULES ON WORKING IN THE MICROBIOLOGY LABORATORY<br \/>\nA Microbiology laboratory is a suitable place that is fitted out to handle and examine microorganisms. This type of work must be carried out with a good aseptic technique, and therefore a clean and tidy environment is required and work must always be carried out in sterile [&#8230;]<\/p>\n","protected":false},"author":3782,"featured_media":0,"parent":0,"menu_order":50,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"_links_to":"","_links_to_target":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/docenciamicrobiologia.umh.es\/en\/12-preparacion-de-yogur\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Safety Rules - MicroWeb UMH\" \/>\n<meta property=\"og:description\" content=\"GENERAL RULES ON WORKING IN THE MICROBIOLOGY LABORATORY A Microbiology laboratory is a suitable place that is fitted out to handle and examine microorganisms. 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