{"id":221,"date":"2021-11-05T13:21:56","date_gmt":"2021-11-05T12:21:56","guid":{"rendered":"https:\/\/docenciamicrobiologia.umh.es\/?page_id=221"},"modified":"2024-10-02T09:15:20","modified_gmt":"2024-10-02T07:15:20","slug":"microbiota-de-piel","status":"publish","type":"page","link":"https:\/\/docenciamicrobiologia.umh.es\/en\/indice-de-practicas\/10-microbiota-humana\/microbiota-de-piel\/","title":{"rendered":"Skin microbiota"},"content":{"rendered":"

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Take a sample of the forearm or the area behind the ear with a sterile swab and inoculate half of a plate with a MSA (Mannitol Salt Agar) culture medium, which is a selective culture medium for <\/span>Staphylococcus.<\/span><\/i> Then apply a disinfectant (alcohol) on this area and take another sample with a new swab in the other half of the plate.<\/span><\/p>\n

Incubate everything at 37\u00baC for 24 hours and then observe the type of colonies and see whether the medium has changed color.<\/span><\/p>\n

Staphylococcus aureus<\/span><\/i> grows by forming yellow colonies when the mannitol is used, which produces acid and it changes the color of the culture medium, while the <\/span>Staphylococcus epidermidis<\/span><\/i> does not use mannitol and therefore no color change is observed.<\/span><\/p>\n

The results are shown in the photo below, in which there are growth differences before and after the treatment with alcohol.<\/span><\/p>\n

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The students must also take a sample of under the nails and of the belly button with two sterile swabs (see photos) and inoculate each of the samples on the half of the other MSA plate. <\/span><\/p>\n

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It is also incubated at 37\u00baC for 24 hours and the possible results are observed.<\/span><\/p>\n

Different results in which the presence of both <\/span>S. aureus<\/span><\/i> (yellow color) and <\/span>S. epidermidis (<\/span><\/i>pink color) are shown<\/span>.<\/span><\/i><\/p>\n

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Effect of the antiseptic on the skin biota.<\/span>
\nPhoto: Clara Quinquer<\/td>\n		Effect of the antiseptic on the skin biota<\/span>
\nPhoto: Raquel Almarcha<\/td>\n		Effect of the antiseptic on the skin biota<\/span>.
\nPhoto: Claudia Ali\u00f1o<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

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Apoyo a acciones de Innovaci\u00f3n Docente – Vicerrectorado de Estudios \/ Consultas e incidencias t\u00e9cnicas- Tlf: 96 522 2059 – info@goumh.es <\/a><\/span>Skin microbiota<\/p>","protected":false},"excerpt":{"rendered":"

Take a sample of the forearm or the area behind the ear with a sterile swab and inoculate half of a plate with a MSA (Mannitol Salt Agar) culture medium, which is a selective culture medium for Staphylococcus. Then apply a disinfectant (alcohol) on this area and take another sample with a new swab in […]<\/p>\n","protected":false},"author":3782,"featured_media":0,"parent":212,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"_links_to":"","_links_to_target":""},"yoast_head":"\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\t\n