{"id":107,"date":"2021-11-05T13:01:47","date_gmt":"2021-11-05T12:01:47","guid":{"rendered":"https:\/\/docenciamicrobiologia.umh.es\/?page_id=107"},"modified":"2024-12-11T10:08:30","modified_gmt":"2024-12-11T09:08:30","slug":"prueba-del-o-f-oxidacion-fermentacion","status":"publish","type":"page","link":"https:\/\/docenciamicrobiologia.umh.es\/en\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/","title":{"rendered":"O\/F glucose test (oxidation-fermentation)"},"content":{"rendered":"<p><strong>Overview<\/strong><br \/>\nThe metabolism of a glucide might follow the oxidative or the fermentative pathway. In the oxidative pathway the final electron acceptor must be the oxygen and therefore the process is aerobic and it produces a small amount of acidity, while in the fermentative pathway the final electron acceptor is an organic compound and the process is anaerobic, which produces a lot of acidity in a short amount of time.<\/p>\n<p>The fermentation or the oxidation of the glucide can be seen in two test tubes with a glucoside culture medium, one open (aerobic) and the other one closed with paraffin (anaerobic).<\/p>\n<p>For this test the semisolid Hugh-Leifson medium is used to which glucose is added in order to see whether this sugar is metabolized through the oxidative or fermentative pathway.<\/p>\n<p style=\"text-align: center\"><iframe  id=\"_ytid_22027\"  width=\"480\" height=\"270\"  data-origwidth=\"480\" data-origheight=\"270\" src=\"https:\/\/www.youtube.com\/embed\/iOnYMcHZvPw?enablejsapi=1&autoplay=0&cc_load_policy=0&cc_lang_pref=&iv_load_policy=1&loop=0&modestbranding=0&rel=1&fs=1&playsinline=0&autohide=2&theme=dark&color=red&controls=1&\" class=\"__youtube_prefs__  no-lazyload\" title=\"YouTube player\"  allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen data-no-lazy=\"1\" data-skipgform_ajax_framebjll=\"\"><\/iframe><\/p>\n<p><strong>Carrying out the practical<\/strong><br \/>\nA stab culture is formed by inoculating the semisolid medium of the two test tubes.<\/p>\n<p>A small amount of sterile paraffin is added to one of them, and they are both incubated at 37\u00baC for 24 hours.<\/p>\n<p>If the pathway is oxidative, only the open tube will turn slightly yellow on the top part. If the pathway is fermentative it turns a deep yellow that starts at the bottom of the two test tubes.<\/p>\n<table style=\"border: hidden;margin: 0 auto\">\n<tbody>\n<tr style=\"border: hidden\">\n<td style=\"text-align: center\"><img loading=\"lazy\" class=\"alignnone wp-image-422 size-medium\" src=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792-213x300.png\" alt=\"\" width=\"213\" height=\"300\" srcset=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792-213x300.png 213w, https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792.png 232w\" sizes=\"(max-width: 213px) 100vw, 213px\" \/><\/td>\n<td style=\"text-align: center\"><img loading=\"lazy\" class=\"alignnone size-medium wp-image-1825\" src=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-229x300.jpg\" alt=\"\" width=\"229\" height=\"300\" srcset=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-229x300.jpg 229w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-783x1024.jpg 783w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-768x1005.jpg 768w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-1174x1536.jpg 1174w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-1566x2048.jpg 1566w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Fermentacion-scaled.jpg 1957w\" sizes=\"(max-width: 229px) 100vw, 229px\" \/><\/td>\n<td style=\"text-align: center\"><img loading=\"lazy\" class=\"alignnone wp-image-1737 size-medium\" src=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Oxidacio\u0301n-Fermentacio\u0301n-1-scaled-e1669055499758-141x300.jpg\" alt=\"\" width=\"141\" height=\"300\" srcset=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Oxidacio\u0301n-Fermentacio\u0301n-1-scaled-e1669055499758-141x300.jpg 141w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Oxidacio\u0301n-Fermentacio\u0301n-1-scaled-e1669055499758-482x1024.jpg 482w, https:\/\/docenciamicrobiologia.umh.es\/files\/2022\/11\/Oxidacio\u0301n-Fermentacio\u0301n-1-scaled-e1669055499758.jpg 644w\" sizes=\"(max-width: 141px) 100vw, 141px\" \/><\/td>\n<\/tr>\n<tr style=\"border: hidden\">\n<td style=\"text-align: center\"><span style=\"font-size: 14px\">Recently inoculated medium<\/span><\/td>\n<td style=\"text-align: center\"><span style=\"font-size: 14px\">Oxidative pathway<\/span><\/td>\n<td style=\"text-align: center\"><span style=\"font-size: 14px\">Fermentative pathway<br \/>\nFernando Ram\u00f3n-Borja<br \/>\n&amp; Sergio Cabot<br \/>\n<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 14px\"> Apoyo a acciones de Innovaci\u00f3n Docente &#8211; Vicerrectorado de Estudios \/ Consultas e incidencias t\u00e9cnicas- Tlf: 96 522 2059 &#8211; <a href=\"mailto:info@goumh.es\" target=\"_blank\" rel=\"noopener\">info@goumh.es <\/a><\/span><\/p>","protected":false},"excerpt":{"rendered":"<p>Overview<br \/>\nThe metabolism of a glucide might follow the oxidative or the fermentative pathway. In the oxidative pathway the final electron acceptor must be the oxygen and therefore the process is aerobic and it produces a small amount of acidity, while in the fermentative pathway the final electron acceptor is an organic compound and the process [&#8230;]<\/p>\n","protected":false},"author":3782,"featured_media":0,"parent":83,"menu_order":27,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"_links_to":"","_links_to_target":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/docenciamicrobiologia.umh.es\/en\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"O\/F glucose test (oxidation-fermentation) - MicroWeb UMH\" \/>\n<meta property=\"og:description\" content=\"Overview The metabolism of a glucide might follow the oxidative or the fermentative pathway. In the oxidative pathway the final electron acceptor must be the oxygen and therefore the process is aerobic and it produces a small amount of acidity, while in the fermentative pathway the final electron acceptor is an organic compound and the process [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/\" \/>\n<meta property=\"og:site_name\" content=\"MicroWeb UMH\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/UniversidadMiguelHernandezElche\" \/>\n<meta property=\"article:modified_time\" content=\"2024-12-11T09:08:30+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792-213x300.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@UniversidadMH\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Organization\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#organization\",\"name\":\"Universidad Miguel Hern\\u00e1ndez\",\"url\":\"https:\/\/docenciamicrobiologia.umh.es\/\",\"sameAs\":[\"https:\/\/www.facebook.com\/UniversidadMiguelHernandezElche\",\"http:\/\/instagram.com\/universidadmh\",\"http:\/\/es.linkedin.com\/edu\/universidad-miguel-hern\\u00e1ndez-de-elche-12237\",\"http:\/\/youtube.umh.es\/\",\"https:\/\/twitter.com\/UniversidadMH\"],\"logo\":{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#logo\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/logon.png\",\"contentUrl\":\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/logon.png\",\"width\":300,\"height\":100,\"caption\":\"Universidad Miguel Hern\\u00e1ndez\"},\"image\":{\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#logo\"}},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#website\",\"url\":\"https:\/\/docenciamicrobiologia.umh.es\/\",\"name\":\"MicroWeb UMH\",\"description\":\"Universidad Miguel Hern\\u00e1ndez de Elche\",\"publisher\":{\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/docenciamicrobiologia.umh.es\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#primaryimage\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792.png\",\"contentUrl\":\"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792.png\",\"width\":232,\"height\":326},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#webpage\",\"url\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/\",\"name\":\"O\/F glucose test (oxidation-fermentation) - MicroWeb UMH\",\"isPartOf\":{\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#primaryimage\"},\"datePublished\":\"2021-11-05T12:01:47+00:00\",\"dateModified\":\"2024-12-11T09:08:30+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Portada\",\"item\":\"https:\/\/docenciamicrobiologia.umh.es\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"\\u00cdndice de Pr\\u00e1cticas\",\"item\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"5. Identificaci\\u00f3n Bacteriana\",\"item\":\"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Prueba del O\/F (oxidaci\\u00f3n-fermentaci\\u00f3n)\"}]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/docenciamicrobiologia.umh.es\/en\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/","og_locale":"en_US","og_type":"article","og_title":"O\/F glucose test (oxidation-fermentation) - MicroWeb UMH","og_description":"Overview The metabolism of a glucide might follow the oxidative or the fermentative pathway. In the oxidative pathway the final electron acceptor must be the oxygen and therefore the process is aerobic and it produces a small amount of acidity, while in the fermentative pathway the final electron acceptor is an organic compound and the process [...]","og_url":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/","og_site_name":"MicroWeb UMH","article_publisher":"https:\/\/www.facebook.com\/UniversidadMiguelHernandezElche","article_modified_time":"2024-12-11T09:08:30+00:00","og_image":[{"url":"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792-213x300.png"}],"twitter_card":"summary_large_image","twitter_site":"@UniversidadMH","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Organization","@id":"https:\/\/docenciamicrobiologia.umh.es\/#organization","name":"Universidad Miguel Hern\u00e1ndez","url":"https:\/\/docenciamicrobiologia.umh.es\/","sameAs":["https:\/\/www.facebook.com\/UniversidadMiguelHernandezElche","http:\/\/instagram.com\/universidadmh","http:\/\/es.linkedin.com\/edu\/universidad-miguel-hern\u00e1ndez-de-elche-12237","http:\/\/youtube.umh.es\/","https:\/\/twitter.com\/UniversidadMH"],"logo":{"@type":"ImageObject","@id":"https:\/\/docenciamicrobiologia.umh.es\/#logo","inLanguage":"en-US","url":"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/logon.png","contentUrl":"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/logon.png","width":300,"height":100,"caption":"Universidad Miguel Hern\u00e1ndez"},"image":{"@id":"https:\/\/docenciamicrobiologia.umh.es\/#logo"}},{"@type":"WebSite","@id":"https:\/\/docenciamicrobiologia.umh.es\/#website","url":"https:\/\/docenciamicrobiologia.umh.es\/","name":"MicroWeb UMH","description":"Universidad Miguel Hern\u00e1ndez de Elche","publisher":{"@id":"https:\/\/docenciamicrobiologia.umh.es\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/docenciamicrobiologia.umh.es\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"ImageObject","@id":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#primaryimage","inLanguage":"en-US","url":"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792.png","contentUrl":"https:\/\/docenciamicrobiologia.umh.es\/files\/2021\/11\/EmbeddedImage-18-e1668774337792.png","width":232,"height":326},{"@type":"WebPage","@id":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#webpage","url":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/","name":"O\/F glucose test (oxidation-fermentation) - MicroWeb UMH","isPartOf":{"@id":"https:\/\/docenciamicrobiologia.umh.es\/#website"},"primaryImageOfPage":{"@id":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#primaryimage"},"datePublished":"2021-11-05T12:01:47+00:00","dateModified":"2024-12-11T09:08:30+00:00","breadcrumb":{"@id":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/prueba-del-o-f-oxidacion-fermentacion\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Portada","item":"https:\/\/docenciamicrobiologia.umh.es\/"},{"@type":"ListItem","position":2,"name":"\u00cdndice de Pr\u00e1cticas","item":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/"},{"@type":"ListItem","position":3,"name":"5. Identificaci\u00f3n Bacteriana","item":"https:\/\/docenciamicrobiologia.umh.es\/indice-de-practicas\/5-identificacion-bacteriana\/"},{"@type":"ListItem","position":4,"name":"Prueba del O\/F (oxidaci\u00f3n-fermentaci\u00f3n)"}]}]}},"_links":{"self":[{"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/pages\/107"}],"collection":[{"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/users\/3782"}],"replies":[{"embeddable":true,"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/comments?post=107"}],"version-history":[{"count":0,"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/pages\/107\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/pages\/83"}],"wp:attachment":[{"href":"https:\/\/docenciamicrobiologia.umh.es\/en\/wp-json\/wp\/v2\/media?parent=107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}