Filamentous fungi and yeast culture medium

Set up filamentous fungi and yeast culture media in Sabouraud agar.

A. Of yeast.- TTake a sample of a yeast colony and set up a subculture in Sabouraud agar using an isolation technique. To be precise streak it as explained in the accompanying figure. Incubate at room temperature (in the laboratory cupboard).

Quadrant streak technique. Obverse Quadrant streak technique. Reverse

B. Of filamentous fungi.- Take a fragment of a colony of one of the filamentous fungi supplied (Aspergillus;. Fusarium; Alternaria; Cladosporium) of approximately 0.5 x 0.5 mm and transfer it to the center of a Petri dish with Sabouraud agar. 3 equally spaced pricks can also be made. Incubate at room temperature (in the laboratory cupboard).

Obverse Reverse

 

Inoculation in 3 points. Obverse Inoculation in three points. Reverse Inoculation in three points. Obverse Inoculation in three points. Reverse

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